Scarless conditional sgRNAs via endogenous mascRNA processing enable rapid and temporally controlled genome editing
A scarless conditional sgRNA platform that combines Cre-loxP recombination with endogenous RNA processing to restore the native sgRNA architecture following induction is developed that preserves guide integrity and should be readily adaptable to time-resolved functional genomics and pooled screening applications.
Curtis Hart, Lovely Paul Solomon Devakumar, Khalid Saeed et al.
· bioRxiv · 0 citations