Prolyl endoprotease (An-PEP) for hydrogen-deuterium exchange mass spectrometry and protein structural studies
Due to its Pro-preference and resistance to low pH, An-PEP is an archetype enzyme for HDX-MS, and higher sequence resolution could be achieved, especially for Pro-containing protein regions in the alpha subunit of Hb, revealing new protected Hb regions that were not observed with pepsin.
L. Tsiatsiani, M. Akeroyd, M. Olsthoorn et al.
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