Characterisation and In Vitro Biocompatability of Polycaprolactone: Cissus Quandrangularis Electrospun Scaffold for Bone Regeneration
The aim of the present study is to develop an osteogenic bone graft material with an herbal component. Cissus quadrangularis, a perennial climber widely used in traditional Indian is reported to possess bone fracture healing activity. Polycaprolactone (PCL) an FDA approved biocompatible polymer and Cissus Quadrangularis (CQ) were blend and electrospun to obtain a nanofibrous scaffold. The fiber morphology was characterised by scanning electron microscopy, FT-IR, XRD and TGA. MG63 osteoblast-like cells were used to evaluate the biocompatibility using MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. The in-vitro experiment revealed that the PCL-CQ composite electrospun scaffold showed better MG63 cell adhesion and growth compared to the control PCL scaffold and thus could be a potential scaffold material for bone defect applications.