The in-vitro experiment revealed that the PCL-CQ composite electrospun scaffold showed better MG63 cell adhesion and growth compared to the control PCL scaffold and thus could be a potential scaffold material for bone defect applications.
Abstract
The aim of the present study is to develop an osteogenic bone graft material with an herbal component. Cissus quadrangularis, a perennial climber widely used in traditional Indian is reported to possess bone fracture healing activity. Polycaprolactone (PCL) an FDA approved biocompatible polymer and Cissus Quadrangularis (CQ) were blend and electrospun to obtain a nanofibrous scaffold. The fiber morphology was characterised by scanning electron microscopy, FT-IR, XRD and TGA. MG63 osteoblast-like cells were used to evaluate the biocompatibility using MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. The in-vitro experiment revealed that the PCL-CQ composite electrospun scaffold showed better MG63 cell adhesion and growth compared to the control PCL scaffold and thus could be a potential scaffold material for bone defect applications.
The scaffold showed the highest osteoinduction, and the scaffold with 530 ± 56 μm average pore diameter demonstrated the highest expression of osteodifferentiation marker genes in DPSCs, and the addition of nanoparticles into the polymer matrix led to the decrease in the expression of pro-inflammatory genes in macropha...
A. V. Yushkov, E. A. Kuvshinova, I. Bulygina et al.· Biomedical Materials· 0 citations
The findings support scaffold feasibility and localized delivery, not proven HO prevention, and further disease-model efficacy, biological activity, dose optimization, degradation, and safety studies are required before clinical translation.
Chih-Yang Lai, Po-Ju Lai, Szu-Yao Wang et al.· Materials Science in Additiv...· 0 citations
3D scaffolds based on Chitosan/Collagen/Poly(lactic-co-glycolic acid) (PLGA)/Hydroxyapatite (HAp) nanoparticles are presented as a promising approach to studying the migration of the 3T3 cell line and demonstrate promising effects on fibroblast migration in an in vitro scratch-assay model using NIH 3T3 cells.
Laila Procel-Badillo, Sarah Briceño, Lenin Ramírez et al.· Polymers· 0 citations
The results showed that the composite scaffolds effectively improved the inflammatory microenvironment in the defect region, promoted macrophage polarization toward an anti-inflammatory phenotype, enhanced cellular osteogenic activity and mineralization, and facilitated new bone formation and tissue reconstruction.
Fang Tong, Ting-Ting Lu, Lu Tang et al.· Journal of materials chemist...· 0 citations
The synthesis of biodegradable nanofibers composed of polycaprolactone, chlorophyllin sodium copper, and polyvinyl acetate via electrospinning for scaffold development in TE is examined to highlight the potential of PCL/CSC/PVAc composite nanofibers as scaffolds for TE applications by providing favorable fiber morpholo...
Muhammad Zikri Aiman Zulkifli, Darman Nordin, N. Shaari et al.· Polymer Bulletin· 0 citations
These findings underscore the dual function of rGO in simultaneously improving the mechanical integrity and osteogenic capacity of CMC-based scaffolds, with 0.5%-1% rGO identified as the optimal concentration window for bone tissue engineering applications.
Ronghui Zhou, Yan-Jun Lin, Xiaojing Zhu et al.· Journal of Biomedical Materi...· 0 citations
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