Ribosome engineering enhances genetic code expansion in Saccharomyces cerevisiae
Genetic code expansion enables the site-specific installation of noncanonical amino acids (ncAAs) into proteins, but its limited efficiency in eukaryotes remains a major barrier to broader application. Here we establish a visual, plug-and-play screening platform to evolve 18S ribosomal DNA in Saccharomyces cerevisiae a...
Xiao-Xu Chen, Wentao Shen, Xian-Qing Chen et al.
· Synthetic and Systems Biotec... · 0 citations