Universal donor plasmids for biallelic degron-tagging in mammalian cells and organoids
A series of universal donor vectors are generated to enable biallelic integration of different degron tags using different selection strategies (FACS, drug selection) and can be fused in-frame to endogenous protein C-termini via homology-independent repair following Cas9-induced double strand breaks.
S. Ö. Pohl, Brianda Hernandez Moran, Camila Guzman Cardozo et al.
· bioRxiv · 0 citations