Affinity Engineering of a Hyaluronic Acid-Specific Carbohydrate-Binding Module Using a Rule-Constrained Large Language Model Agent
Hyaluronic acid (HA) is widely used in food and nutritional supplements, yet reliable quantification in complex matrices remains limited by the lack of cost-effective, high-affinity recognition elements. Carbohydrate-binding modules (CBMs) are stable, recombinantly accessible, and intrinsically selective for polysacc...