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Curtis Hart

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Open access Aug 2026

Comparative Single-Cell Profiling of CRISPR Knockout and Interference Defines Modality-Specific Strengths in Functional Genomics

While CRISPRi allows direct assessment of target gene repression, CRISPRko provides an effective complementary approach for complete loss-of-function studies, expanding the toolkit for single-cell functional genomics.

Nikhil Gupta, Andrew Sayer, Malwina Prater et al. · 0 citations
#gene editing Open access Aug 2026

Scarless conditional sgRNAs via endogenous mascRNA processing enable rapid and temporally controlled genome editing

A scarless conditional sgRNA platform that combines Cre-loxP recombination with endogenous RNA processing to restore the native sgRNA architecture following induction is developed that preserves guide integrity and should be readily adaptable to time-resolved functional genomics and pooled screening applications.

Curtis Hart, Lovely Paul Solomon Devakumar, Khalid Saeed et al. · 0 citations

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