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Da-Feng Liu

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#protein folding Open access Nov 2026

The Surrogate Substrate p-Nitrophenyl Phosphate (pNPP) for the Phosphatidylinositol-3-Phosphatase SapM from Mycobacterium tuberculosis: Mutational Analysis of pNPP Hydrolysis and Preliminary Single-Gating Observations

The findings provide a molecular framework for SapM function and identify segment 237–249 as a candidate regulatory element for future studies, and whether SapM can be targeted therapeutically remains to be established.

Da-Feng Liu, Wen-Shuang Yao · 0 citations
Open access Aug 2026

Functional and mechanistic characterization of the phosphatidyl-myo-inositol mannosyltransferase PimA from Mycobacterium tuberculosis: implications for anti-tuberculosis drug development

Human-adapted Mycobacterium tuberculosis (Mtb) also infects animals and transmits zoonotically, threatening public health. The cell wall glycolipids lipoarabinomannan and lipomannan are essential virulence factors of Mtb. Phosphatidyl-myo-inositol mannosyltransferase PimA catalyzes a key step in their biosynthesis and...

Da-Feng Liu, Hua-Shui Deng, Xinbao Liu et al. · 1 citation
Open access Sep 2026

The Flexible Peripheral Segments Modulate the Catalytic Activity of the FAD-Containing Monooxygenase EthA from Mycobacterium tuberculosis

An AlphaFold2-predicted structural model of Mtb EthA is reported and it is suggested that flexible peripheral regions may constrain catalytic activity, offering insights into ETH activation and potential mechanisms of drug resistance.

Da-Feng Liu, Xie-Ping Sun, Feng Yu et al. · 0 citations
Open access Sep 2026

The Dephospho-CoA Kinase of Mycobacterium tuberculosis: Molecular Mechanism and Function

The found that the S15A, R145A or N175A mutants showed markedly reduced activity, whereas the K14A mutation completely abolished activity, and deletion of residues 27-101 (Δ27-101) led to a significant loss of function.

Da-Feng Liu, Hua-Shui Deng, Hong-Jun Song · 0 citations

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