Analysing long-read CRISPR experiments with CRISPRLungo.
This work presents CRISPRLungo, a computational pipeline specifically designed for long-read amplicon sequencing of gene edited samples that incorporates unique molecular identifier-based error correction and statistical filtering to distinguish true editing events from background noise, enabling robust detection of small indels and structural variants.
Gue-Ho Hwang, Benjamin Vyshedskiy, Timothy M. Barry et al.
· Nature Biomedical Engineerin... · 0 citations