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E. Buslaeva

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#protein folding Aug 2026

ENGINEERING 3'-UTR HAIRPIN STRUCTURES TO MODULATE MRNA STABILITY AND RECOMBINANT PROTEIN PRODUCTION IN ESCHERICHIA COLI.

Engineering mRNA stability is a promising yet underexplored approach for improving recombinant protein production in bacterial systems. In this study, we evaluated the effect of synthetic 3'-UTR hairpin structures on mRNA stability and protein yield in Escherichia coli using two SUMO-fusion expression systems. Hairpin...

Z. Khasanshina, M. Yarovikova, E. Buslaeva et al. · 0 citations
Aug 2026

An expression framework for production of recombinant methionine aminopeptidase in Escherichia coli: a case study of upstream process.

A case-study evaluation of a pBR322-derived expression system, previously applied to therapeutic peptides and insulin analogs, in combination with fed-batch cultivation for recombinant production of methionine aminopeptidase in E. coli, evaluating the performance of this system for methionine aminopeptidase as a stress...

G. Kuznetsov, Marina Yarovikova, E. Buslaeva et al. · 0 citations

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