Author

Eamonn Reading

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#protein folding Open access Sep 2026

Droplet Microfluidic Hydrogen/Deuterium Exchange for Investigating Protein Dynamics with Millisecond Precision

Hydrogen/Deuterium eXchange (HDX) methods for studying protein dynamics would benefit from millisecond-scale incubations to probe intrinsically disordered proteins, highly dynamic regions, and conformation changes. Here, we investigate droplet microfluidics for rapid mixing to trigger D2O labeling, uniform incubation...

Dietmar Hammerschmid, Jakub Sýs, A. Bailey et al. · Analytical Chemistry · 1 citation

#protein folding Open access Aug 2026

LemonCatcher Acidic Pull-Down Enables Selective In-Cell Hydrogen-Deuterium Exchange Mass Spectrometry

LemonCatcher is created, a protein superglue that spontaneously forms an amide bond to the LemonTag peptide under these harsh acidic and cold quench conditions, even at -20 °C, and SelQueX makes possible selective characterization of protein structural dynamics within the complex cellular milieu.

Dietmar Hammerschmid, Mahjoobeh Eshani, A. H. Keeble et al. · bioRxiv · 0 citations