Generation of Quarter- and Hemi-embryos via blastomere isolation reduces mosaicism and reveals editing patterns in Cas9 RNP-edited bovine embryos
Mosaicism is a persistent limitation in CRISPR/Cas9-based genome editing of preimplantation embryos. This study tested whether isolating blastomeres at the 2- or 4-cell stage reduces mosaicism in the resulting blastocysts and used the resulting variant patterns at the target region to analyze the duration of activity o...
M. Rahimi, H. Habermann, L. Haake et al.
· Scientific Reports · 0 citations