A cryptic binding pocket regulates the metal-dependent activity of Cas9
Abstract Cas9 is a metal-dependent nuclease used for genome editing across diverse cells and organisms exhibiting distinct ionic environments, yet how metal ions regulate its catalytic function, and consequently its editing efficiency remains unclear. Here, molecular simulations, Markov state models, and mixed quantum–...
Mohd Ahsan, Alexa L Knight, Aakash Saha et al.
· Nature Communications · 2 citations