Aug 2026
Quantitative profiling of intrinsic dCas9-DNA recognition reveals key determinants of guide RNA performance
TANGO (Targeted Array-based Nucleic acid-Guided Occupancy), a high-density DNA-array platform that quantitatively profiles intrinsic dCas9:gRNA binding across tens of thousands of DNA targets in a cell-free system, establishes intrinsic DNA recognition as a quantitative and experimentally accessible determinant of dCas9 function.
Wei Zhu, Michael Tian, Yuncheng Duan et al.
· bioRxiv · 0 citations