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The transcript-aware Whole-genomic Evaluation for Editing Locus is presented, a pipeline that infers redundancy in silico from transcript information and cross-references it with specific guide RNA designs to compute a knockout feasibility index (KFI) for individual genes and functional categories.
The results suggest that the genome data were of sufficient quality for use as a reference genome and can be utilized as a genomic resource for comparative studies within Braconidae and for future investigations into the biology and evolution of Cheloninae parasitoid wasps.
MicroRNAs (miRNAs) play essential roles in posttranscriptional regulation of gene expression. During miRNA biosynthesis, miRNA precursors are cleaved by Dicer at their loop structure to generate mature miRNAs. However, the functional consequences of sequence alterations in the loop region remain poorly understood. The...
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