Author

H. Habermann

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#gene editing Open access Nov 2026

Generation of Quarter- and Hemi-embryos via blastomere isolation reduces mosaicism and reveals editing patterns in Cas9 RNP-edited bovine embryos

Mosaicism is a persistent limitation in CRISPR/Cas9-based genome editing of preimplantation embryos. This study tested whether isolating blastomeres at the 2- or 4-cell stage reduces mosaicism in the resulting blastocysts and used the resulting variant patterns at the target region to analyze the duration of activity o...

M. Rahimi, H. Habermann, L. Haake et al. · Scientific Reports · 0 citations

Open access Sep 2026

Zygote origin shapes sensitivity to electrical voltage field and editing susceptibility after CRISPR/Cas9-RNP electroporation in bovine embryos.

Despite major progress in generation of edited embryos and offspring via CRISPR/Cas9-RNP electroporation, editing outcomes remain highly variable. This variability may be due to intrinsic differences between in vivo-derived (IVV) and in vitro-derived (IVT) zygotes, which may play a more decisive role than previously an...

M. Rahimi, D. Miskel, M. Schreiber et al. · Theriogenology · 0 citations