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Hong-Li Zhou

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#protein folding Sep 2026

Ultrasound-Synchronized In Situ Crystallization Locks Transient High-Activity Enzyme Conformations within Covalent Organic Frameworks

Enzymes transiently access high-activity conformations with exposed cryptic sites and heightened substrate affinity, yet these states dissipate upon relaxation to the thermodynamic ground state once the activating perturbation is removed, leaving this catalytic potential largely inaccessible. Here we show that such f...

Hong-Li Zhou, Shuang Chen, Hai-Yue Zhou et al. · 0 citations
Open access Jul 2026

Maximizing the productivity of co-transcriptional in vitro transcription

An optimized co-IVT process that achieves a yield of more than 26 g/L while maintaining capping efficiency of over 99.5%, and achieving a 30-fold reduction in double-stranded RNA (dsRNA), the most critical process-related impurity, substantially improves mRNA production efficiency and enhance mRNA quality, thereby acce...

Wei He, Kun-Kun Zhang, Guiying Ji et al. · 0 citations

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