The rapid expansion of human genomic data has revealed a large number of naturally occurring variants, creating a major challenge for functional annotation. The human metal transporter SLC39A8 (ZIP8) is a clinically important, promiscuous divalent metal transporter, yet most of its documented variants remain uncharacte...
Michael Nikolovski, Tian-Qi Wang, Aaron Sue et al.· bioRxiv· 0 citations
Biosynthesis of the nickel-pincer mononucleotide metallocofactor requires a CTP-dependent nickel insertion reaction catalyzed by LarC, whose mechanism of C-Ni bond formation is not fully understood. Here, we report the first cryo–electron microscopy structures of full-length LarC from Moorella thermoacetica with and wi...
Robert Wolfe, Aiko Turmo, Samantha Velasquez-Rivertte et al.· Science Advances· 0 citations
A workflow to functionally evaluate ZIP8 variants by integrating laser ablation inductively coupled plasma time-of-flight mass spectrometry with scaled-up cell-based transport assays is developed and provides new insights into the structure-function relationships of ZIP8.
Michael Nikolovski, Tian-Qi Wang, Aaron Sue et al.· Journal of Biological Chemis...· 0 citations
This study demonstrates that combining internal symmetry analysis and ASR for the ZIP family facilitates elucidation of the metal translocation mechanism and identification of subfamily‐specific features that regulate transport function.
Yao Zhang, Tian-Qi Wang, Hong-Yan Zhao et al.· Protein Science· 0 citations
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