Systematic Screening of Reference Genes for qPCR in Procambarus clarkii: RPL13a Shows Stable Expression Under Non-Ionic Ammonia Stress and Multiple Experimental Conditions
Real-time quantitative PCR (qPCR) is a key technique for elucidating the mechanisms underlying environmental stress responses in aquatic animals, and its accuracy relies heavily on stable reference genes for data normalization. In this study, histopathological examination revealed alterations in the gill, hepatopancrea...