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Open access Sep 2026

Engineered Escherichia coli Nissle with an Inducible Phenylalanine Degrading Cassette and a Multi-input Kill Switch.

Engineered probiotics provide new avenues for disease management through diagnostic sensing, delivery of bioactive molecules, and modulation of host-microbiome interactions. However, successful clinical translation requires rational safeguards preventing unintended environmental release. We report an integrated phenyla...

Esse M. Evbuomwan, Sakshi Khanna, Cheng-Gang Xi et al. · 0 citations
#protein folding Sep 2026

A combinatorial construct library enables an expanded expression range of secreted therapeutic proteins by probiotic yeast.

A 125-plex Sb secretion construct library was developed consisting of all possible combinations of five promoters, five secretion signals, and five terminators, which enabled a greater than 1800-fold range in Sb expression of a Gaussia luciferase (GLuc) reporter.

Miranda J. Wallace, Christina Kratschmer, Brandon Brazil et al. · 0 citations

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