pH-Responsive Hierarchical Self-Assembly of Engineered Protein–Gold Nanocluster Conjugates with Tunable Fluorescence
We report the rational engineering of a two-α-helix protein via site-directed mutagenesis, wherein histidine residues at the native catalytic active site were substituted with cysteines and tyrosine residues were introduced in close proximity to these cysteine residues. This modification enabled the site-directed red...