Aug 2026· ACS Applied Nano Materials· 1 citation· 50 references
Abstract
We report the rational engineering of a two-α-helix protein via site-directed mutagenesis, wherein histidine residues at the native catalytic active site were substituted with cysteines and tyrosine residues were introduced in close proximity to these cysteine residues. This modification enabled the site-directed reduction and nucleation of free gold ions into size-tunable gold nanoclusters (AuNCs) exhibiting red, green, and blue photoluminescence upon ultraviolet excitation. Furthermore, by modulating the solution pH, we successfully fabricated nanofibers via the co-assembly of the protein and AuNCs. Comprehensive structural characterization confirmed the architecture of these biohybrid materials. Protein-AuNCs can catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB), exhibiting peroxidase-like activity under specific conditions. Furthermore, this peroxidase-like activity was successfully applied to the colorimetric detection of glutathione with a linear response in the concentration range of 100–300 μM.
This study presents a rational, simulation-guided strategy to overcome the structural instability of ferritin nanocages upon conjugation with large biomoleculesa common challenge in protein-based bioconjugates. Molecular dynamics (MD) simulations were performed using epidermal growth factor (EGF) and enhanced green...
Sensitive and accurate monitoring of arginase activity is significant for clinical diagnosis. Herein, a dual-enhanced aggregation-induced electrochemiluminescence (ECL) biosensor was constructed based on triple-ligand-protected gold nanoclusters (AuNCs), sequentially coating 6-aza-2-thiothymine-capped AuNCs with L-argi...
Li-Li Tong, Xue-Ke Luo, Yan Zhang et al.· In Analysis· 0 citations
In this work, a green and sustainable hydrothermal approach was employed to synthesize nitrogen-doped carbon dots (NCDs) using lemon juice as a natural precursor. The as-prepared NCDs served as eco-friendly reducing agents and stabilizing scaffolds for the subsequent in situ synthesis of gold nanoparticles (Au@NCDs). T...
T. Nguyen, V. Nguyen, Ha Thi Thu Nguyen et al.· Beilstein Journal of Nanotec...· 0 citations
This work presents the rational synthesis of linker-engineered cerium-based metal-organic frameworks (Ce-TMA and Ce-TPA) via a facile room-temperature route and elucidates their structure-dependent multifunctional performance in fluorescence sensing, electrochemical detection, and nanozyme catalysis. Ce-TMA nanorods ex...
In this work, red-fluorescent silver nanoclusters (MMTA@Ag NCs) with aggregation-induced emission (AIE) properties were synthesized via a facile one-pot strategy at room temperature using 2-mercapto-4-methyl-5-thiazoleacetic acid (MMTA) as the capping ligand. The as-prepared Ag NCs initially exhibited weak fluorescence...
Chirality is crucial for the function of biological molecules and various chemical reactions, yet recognizing and detecting it remains a significant challenge. R-BITOP, a Lysine enantiomeric-selective fluorescence probe with AIE Properties was efficiently synthesized via nucleophilic cyclization. Upon reaching a water...
Zhuhua Wang, Zhongduo Luojiang, Rong Wang et al.· Spectrochimica Acta Part A -...· 0 citations
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