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Jordan D. Ward

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#gene editing Open access Oct 2026

A systematic fluorescently tagged protein toolkit for dissecting apical extracellular matrix patterning in C. elegans.

Barrier epithelia are shielded from the external environment by their apical extracellular matrices (aECMs). The molecular complexity of aECMs has challenged understanding of their organization in vivo. To define the molecular architecture of a model aECM we generated a toolkit of 105 fluorescently tagged aECM componen...

J. M. Ragle, Murugesan Pooranachithra, Guinevere A. Ashley et al. · PLoS Biology · 0 citations

Sep 2026

An F2A sequence permits correct localization of a secreted and a nuclear localized reporter in C. elegans

Multicistronic expression systems enable production of multiple proteins from a single transcript, with internal ribosome entry sites (IRES), SL2 trans-splicing, and 2A peptides as common tools. Because 2A peptides rely on a single translation event, we tested whether nuclear-localized mStayGold (mSG::H2B) and secreted...

J. M. Ragle, Guinevere A. Ashley, Jordan D. Ward · microPublication Biology · 0 citations

2026

Protein Depletion in Caenorhabditis elegans Using the Auxin-Inducible Degradation System.

This work describes how to generate degron knock-ins by CRISPR, cross them to TIR1-expressing strains, and perform depletion experiments, and provides a description of the current methods and highlight alternative approaches.

Jordan D. Ward · Methods in molecular biology · 0 citations