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By data-mining very large datasets, this study establishes that rather low-abundant events can be analyzed by cryo-ET and paves the way toward broader applications.
A protocol to generate mature forebrain organoids from mouse embryonic stem cells is optimized and time-resolved proteomics is used to define the emergence of synaptic proteins during maturation to demonstrate that complex mature synaptic molecular architecture is present within an in vitro organoid environment.
Neural organoids represent a promising approach for modelling neural circuits and neurological disease, yet their utility depends on whether they can recapitulate the molecular complexity of mature brain synapses. Here, we optimized a protocol to generate mature forebrain organoids from mouse embryonic stem cells and u...
ABSTRACT Recombinant biomacromolecules have significantly transformed pharmaceuticals, in particular monoclonal antibodies (mAbs) that target key areas in oncology, immunology, inflammation, and neurodegenerative diseases. Developing biotherapeutics requires a detailed understanding of their biological properties. This...
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