Splicing-guaranteed artificial introns enable efficient homology-directed repair at internal exons and streamlined cell engineering
Selection-marker-assisted homology-directed repair (HDR) efficiently edits coding sequence termini but is poorly suited for internal exons. Here, we developed artificial reporter intron-based HDR (ARI-HDR), which inserts a selectable intron into target exons to enable precise internal-exon editing. Although designed fo...
Shang Wang, Xianteng Wang, Mengyu Shang et al.
· bioRxiv (Cold Spring Harbor... · 0 citations