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Kazuki Nakamae

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#gene editing Open access Oct 2026

tWhEEL: a transcript-aware workflow for quantifying CRISPR knockout feasibility using transcriptome assembly

The transcript-aware Whole-genomic Evaluation for Editing Locus is presented, a pipeline that infers redundancy in silico from transcript information and cross-references it with specific guide RNA designs to compute a knockout feasibility index (KFI) for individual genes and functional categories.

Kazuki Nakamae, H. Bono · 0 citations

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