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Activity-normalized prime editing provides a scalable framework for LDLR variant classification that substantially expands the proportion of variants with evidence for genetic diagnosis and reveals novel biology with therapeutic relevance.
This work presents CRISPRLungo, a computational pipeline specifically designed for long-read amplicon sequencing of gene edited samples that incorporates unique molecular identifier-based error correction and statistical filtering to distinguish true editing events from background noise, enabling robust detection of sm...
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