Engineered genomic attachment sites for site-specific recombinases enable high-efficiency integration in plants and human cells.
Here we developed a DNA-centric strategy for optimizing site-specific recombination by rationally engineering chimeric attachment sites. The high-activity att variants enhance Bxb1-mediated integration efficiency in human cells and plants. Among these att variants, the engineered attB(V111) site achieved 51.9% integrat...
Lin-Li Yan, Ling Zhou, Qiang Gao et al.
· Nature Biotechnology · 0 citations