A strategy for genome-wide seamless tagging of human protein-coding genes
A universal and scalable method based on CRISPR/Cas9-non-homologous end joining (NHEJ) for Pooled Recombinant Integration of Seamless Markers (PRISM) into protein coding genes, paving the way for genome-scale construction of human cell lines tagged with different types of reporter genes for protein functional character...
Lidice González, Minoo Karimi, Xavier Castellanos-Girouard et al.
· bioRxiv · 0 citations