Complex folding kinetics of multi-domain proteins disentangled with periodic forces by magnetic tweezers
Force spectroscopy enables direct investigation of the interplay between free energy and transition kinetics in proteins and nucleic acids. However, multi-domain proteins often display multiple intermediate structures with diverse stabilities and kinetics, making equilibrium conditions difficult to achieve and out-of-e...
C. A. Quintana-Cataño, Andreas Hartmann, M. Schlierf
· bioRxiv · 0 citations