Maria Zacharopoulou

A generalizable method for the purification and biophysical characterization of bacterial membrane receptors.

Membrane-embedded bacterial receptors are challenging to express and purify in soluble form, yet their isolated domains are essential tools for structural and ligand-discovery studies. Pseudomonas aeruginosa relies on the TonB-dependent heme receptor HasR for iron acquisition, a process central to its pathogenicity. He...

Programmable DNA-peptide nanostructures for multivalent regulation of intracellular signalling

Silane-Enriched Conducting Polymer Thin Films Improve Supported Lipid Bilayer Formation.

Supported lipid bilayers (SLBs) on conductive polymer devices provide a powerful platform for studying membrane processes, enabling cost-effective, label-free, high-throughput bioelectronic sensing. However, their reproducible formation, particularly from native membrane components, remains challenging. Here, we invest...

A generalisable method for the purification and biophysical characterisation of bacterial membrane receptors

A generalizable workflow for producing soluble receptor domains from membrane proteins is established and validated HasR constructs suitable for downstream ligand-screening applications, including aptamer and nanobody discovery.