Oligo-Modified Poly(β-Amino Ester)-Mediated mRNA Delivery: A Protocol for Gene Editing Applications.
A protocol for preparing OM-pBAE nanoparticles is described, including polymer synthesis, peptide modification, controlled mixing with nucleic acids to form polyplexes, precipitation in RNase-free water, stabilization with excipients, and optional freeze-drying for long-term storage.
Laia Martí Melero, Sara Pérez Martín, Salvador Gomez et al.
· Methods in molecular biology · 0 citations