An inducer-independent, single-plasmid CRISPR-Cas9 system for genome editing in Bacillus species
A one-step CRISPR-based transformation protocol developed in this study enables fast genome editing workflows with minimal hands-on time and demonstrates the versatility of the modified system for this industrially important genus.
Maximilian Hilkmann, Norma Welsch, M. F. Felle et al.
· Applied Microbiology and Bio... · 0 citations