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Author

Meredith Frie

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#gene editing Open access Oct 2026

Knockout and re-expression system for mutant analysis in primary mouse T cells

We describe here an efficient method for gene editing in mouse T cells, based on well-established, high-efficiency retroviral transduction protocols. Our platform allows analysis of mutant phenotypes in primary murine T cells in vitro and in vivo . This approach uses a single retroviral vector to simultaneously knockou...

Vasileios Morfos, Meredith C. Frie, Daniel Peschkov et al. · 0 citations
#gene editing Open access Aug 2026

Knockout and re-expression system for mutant analysis in primary mouse T cells

The knockout and re-expression platform provides a useful gene editing tool for primary T cells in fundamental research and immunotherapy development and can be used to generate gene-edited primary T cells for in vivo studies, such as the quantification of anti-tumor responses.

Vasileios Morfos, M. Frie, Daniel Peschkov et al. · 0 citations

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