An improved protocol for high-efficiency and cost-effective CRISPR/Cas9-mediated knock-ins in C. elegans
CRISPR/Cas9-mediated homology-directed precise genome editing using long single-stranded DNA (ssDNA) donors has expanded the possibilities for generating defined genetic modifications. However, the required ssDNA donor preparation can be technically demanding and often requires extensive locus-specific sequence design....
Atal Vats, Arunima Sen, A. Bandyopadhyay et al.
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