Chemically composed Cas9 (ChemComCas9) for spatio-temporal regulation and precision genome engineering applications.
Chemically composed Cas9 (ChemComCas9) has emerged as a highly versatile CRISPR enzyme, offering tunable, on-demand, and targeted control over genome engineering functions. Despite its programmable, sequence-specific activity, uncontrolled or prolonged exposure to Cas9 can lead to severe genotoxicity, restricting its e...