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A novel regulatory module is revealed, MsDREB1C–MsPUB6, in which a DREB1 transcription factor positively regulates a U-box-type E3 ligase gene to contribute to drought tolerance in alfalfa.
ABSTRACT Drought constitutes a major abiotic stress limiting alfalfa productivity. To elucidate the genetic basis of drought tolerance, a genome‐wide association study (GWAS) was conducted in alfalfa, which identified a CBF/DREB1 family transcription factor designated MsCBF8. Utilizing an optimized CRISPR‐Cas9 system,...
The first genome-wide analysis of these gene families in autotetraploid alfalfa provides a theoretical foundation for future functional studies of RNA silencing pathway genes and offer valuable genetic resources for the molecular breeding of stress-resilient alfalfa.
This study generated 528 RNA‐seq libraries from 176 alfalfa accessions under well‐watered and salt stress conditions and functionally validated MsRD26, a NAC transcription factor, as a major candidate positive regulator of salt tolerance.
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