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Sponsored AI Networking Cookbook: Practical recipes for AI-assisted network automation and development AI Networking Cookbook: Practical recipes for AI-assisted network automation and development

Author

R. F. Ocampo

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Open access Sep 2026

Architecture of a DNA-guided Cas12a.

CRISPR-Cas systems have largely been restricted to RNA-guided nucleases. Here, we present the cryo-electron microscopy structure of Acidaminococcus sp. Cas12a bound to a pseudo-DNA (ΨDNA) guide and RNA target, revealing how Cas12a can accomplish DNA-guided RNA recognition. The ΨDNA hairpin bridges the recognition and n...

R. F. Ocampo, Carlos Orosco, Bo-Yu Huang et al. · 0 citations
Open access Jul 2026

CRISPR RNA-independent activation of Cas12a

It is shown that short RNAs can directly occupy the canonical crRNA-binding channel and trigger a catalytically competent trans cleavage state in the absence of PAM recognition or canonical R-loop formation.

I. Iwe, S. Singh, K. Guan et al. · 0 citations

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