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CRISPR RNA-independent activation of Cas12a

I. Iwe S. Singh K. Guan R. F. Ocampo S. J. Ribeiro da Silva D. Wachholz Junior N. Emami A. Corsano I. Zeisler K. Bozovicar L. Wang D. Ham R. Cai P. Kelly R. Zayeni J. Nguyen P. Bayat M. Charania S. Palter F. X. Liu S. Shrestha A. Rayhan G. Wasney T. Mazzulli A. A. Green Z. Li S. Yao B. P. Hubbard D. Taylor K. Pardee
Jul 2026 · medRxiv · 0 citations
Medicine

TL;DR

It is shown that short RNAs can directly occupy the canonical crRNA-binding channel and trigger a catalytically competent trans cleavage state in the absence of PAM recognition or canonical R-loop formation.

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