Author

Ren-Jun Gao

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Review Open access Nov 2026

Integrating noncanonical amino acids and synthetic cofactors for the development of artificial metalloenzymes

Natural metalloenzymes harness the sophisticated synergy between canonical amino acids and metal cofactors to catalyze life-sustaining reactions. However, the limited chemical diversity of the twenty standard amino acids often restricts non-natural transformations. To overcome these constraints, artificial metalloenzym...

Si-Meng Ding, Yu-Xin Shi, Ren-Jun Gao et al. · Communications Chemistry · 0 citations

Open access Oct 2026

Enhancing the flexibility of the peripheral regions within the catalytic cavity of a family 5 glycoside hydrolase CelB for improving catalytic efficiency

Glycoside Hydrolase family 5 (GH5) endoglucanases are indispensable biocatalysts in lignocellulosic biorefining and their intrinsic catalytic performances play vital roles in process economics and enable broader industrial applicability. Herein, we engineered the catalytic domain of a bi-modular GH5 endoglucanase Cel...

Zi-Hao Huang, Si-Meng Ding, Liang-Tao Xu et al. · Frontiers in Bioengineering... · 0 citations

#protein folding Open access Sep 2026

Consensus design and in silico screening of GH42 β-galactosidase for enhanced catalytic hydrolysis of whey lactose.

This study addresses a major bottleneck in dairy waste management by engineering a highly efficient, thermophilic enzyme for lactose hydrolysis, transforming the cheese manufacturing by-product whey into a high-value resource. While traditional glycoside hydrolase family 2 (GH2) enzymes suffer from poor industrial ther...

Si-Meng Ding, Jia-Jia Li, Jia-Hui Li et al. · Journal of Dairy Science · 0 citations