Author

Shi-Hui Yang

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Review Open access Oct 2026

Multi-parameter optimization of recombinant protein secretion in Bacillus subtilis: a review

Bacillus subtilis is widely recognized as one of the most robust and versatile hosts for recombinant protein production. This review provides a comprehensive overview of the protein secretion pathway in B. subtilis, detailing its mechanistic basis and key limiting steps. We discuss the structural features of signal pep...

A. Kholikov, Khushnut Vokhidov, Barno Alimova et al. · Microbial Cell Factories · 0 citations

#gene editing Sep 2026

Highly efficient production of 1,3-propanediol by CRISPR-Cas12a-edited Klebsiella pneumoniae using glycerol and lignocellulosic hydrolysate as co-substrates.

Efficient biosynthesis of 1,3-PDO, a key bio-based chemical, depends on precise regulation of the host metabolic network. In this study, a heterologous CRISPR-Cas12a genome editing system was established and systematically optimized in Klebsiella pneumoniae, enabling efficient and stable genome editing (75-100% efficie...

Li Wang, Ming-Yang Zhao, Yuan-Ming Ye et al. · Bioresource Technology · 0 citations