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c-Jun N-terminal kinases (JNKs) are central integrators of cell stress and are responsible for a multitude of signaling and gene expression changes in response to various environmental cues. Here, we describe the development of JNKAR2, a second-generation FRET-based JNK biosensor. JNKAR2 exhibits a ∼3.9-fold higher res...
Nanoscale protein clustering at the plasma membrane has emerged as a fundamental mechanism for organizing cellular signaling, yet how nanocluster size is actively regulated and whether architectural changes at this scale carry functional consequences remain poorly understood. Here, we used the prototypical membrane sca...
Protein engineering appears to be entering a golden age, defined by a rapidly accelerating pace of progress, even as significant challenges in design, screening, and real-world application remain.
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