PS4-8. Efficient DNA-free Genome Editing in Chicken Cells by Delivering mRNA and Protein Form of cas9.
Genome editing in avian systems has largely relied on plasmid-based CRISPR systems and antibiotic selection to achieve successful editing. However, plasmid delivery can result in prolonged nuclease expression and potential unintended DNA integration. In addition, selection-based enrichment may mask the intrinsic geno...