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These findings establish AF3 as reliable for the prediction of monomer disorder but unpredictable for multimers, confirming that disorder prediction emerges from training data, not model design.
BENDER, a dataset of 11,533 IDP sequences spanning 13 taxonomic groups, each simulated under CALVADOS-2 molecular dynamics and annotated with ensemble-level geometric and novel contact-network properties, suggests that hub topology in disordered protein contact networks is a conserved physical feature of sequence-encod...
Intrinsically disordered proteins and regions are found across all kingdoms of life, yet the computational characterisation of their conformational ensembles has remained almost entirely confined to the human proteome. Whether the physics-based force fields developed on eukaryotic sequences remain reliable for taxonomi...
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