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Author

Tomás Frazão

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#protein folding Open access Oct 2026

Engineering a Modular Bacterial Galactose Oxidase through CBM Recombination and Second-Shell Substitutions

Galactose oxidases (GalOxs) are copper-radical enzymes that selectively oxidize primary alcohols, yet the structure–function determinants governing their catalytic efficiency and substrate scope remain incompletely understood, particularly in bacterial enzymes. Here, we combine biochemical and structural characteriza...

André Taborda, Tomás Frazão, Tiago H. Lopes et al. · 0 citations
Open access Oct 2026

Heme-Proximal Loop Dynamics Modulate Mn(II) Oxidation in Pseudomonas putida MET94 DyP-type peroxidase.

Enzymatic Mn(II) oxidation is relevant to both natural lignin degradation and the development of new industrial biocatalysts. DyP-type peroxidases are versatile microbial biocatalysts with potential applications in lignocellulose valorization, but the structural features influencing their Mn(II)-oxidizing activity rema...

Carolina F. Rodrigues, Constança Lorena, Tomás Frazão et al. · 0 citations

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