An integration-free CRISPR platform for genetic dissection of respiratory virus host dependencies in human nasal epithelium
Respiratory viruses, including coronaviruses, generally initiate infection in the upper respiratory tract, where mechanistic studies of host-virus interactions require models amenable to genetic manipulation. Human nasal air-liquid interface (ALI) cultures are realistic models of the nasal epithelium, but robust CRISPR...
S. W. van Leur, J. Chu, Xue-Sheng Wu et al.
· bioRxiv · 0 citations