LNA-Modified DNAzyme Nanomachines Enable Physiological-Temperature Recognition of Double-Stranded DNA
Sequence-specific detection of double-stranded DNA (dsDNA) under physiological conditions remains challenging because the complementary strand is inaccessible to conventional oligonucleotide probes, often requiring thermal denaturation or protein-assisted strand separation. Here, we developed a binary DNAzyme (BiDz)...