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Author

Wenliang Hao

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Open access Sep 2026

Hijacking host RNA polymerase for processive in situ mutagenesis

Targeted mutagenesis is a crucial tool for in vivo continuous evolution. However, existing methods for long-range targeted mutagenesis in vivo often face limitations such as low efficiency, poor host compatibility, operational complexity, and uneven distribution of mutations across these windows. In this study, we deve...

Yu-Ou Sheng, Hui-Zhen Ni, Shi-Hao Yang et al. · 0 citations
#gene editing Aug 2026

Design of base editing systems with tunable editing hotspots within a defined window through sgRNA engineering.

Base editors (BEs) enable efficient A-to-G or C-to-T conversions without double-stranded DNA cleavage, but their editing windows remain difficult to tune, limiting genome engineering flexibility. Here, we engineered CRISPR/Cas12b sgRNA by introducing MS2 hairpins to recruit an MS2-N55K-cytidine deaminase-UGI complex, e...

Wen-Liang Hao, Lai-Chuang Han, Yao-Kang Wu et al. · 0 citations

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