Combinatorial Optimization of Promoters and Anchoring Proteins Drives a 10-Fold Enhancement in Yeast Surface Display of eGFP
Yeast surface display (YSD) is a powerful tool for protein engineering, yet its broader application is often limited by suboptimal display efficiency. To address this, we systematically investigated the combinatorial effects of three key genetic determinants—promoter strength, anchoring protein identity, and fusion ori...